Products at a glance

Rembrandt®

The wide range of REMBRANDT® products comprise complete kits for the detection of pan Human papilloma virus (HPV screening), HPV typing, Cytomegalo virus (CMV), Herpes simplex virus (HSV) 1/2, Epstein-Barr virus (EBV), Epstein-Barr encoded small mRNA's (EBER) and kappa/lambda light chain mRNA's. All probes are available with either biotin or digoxigenin as choice of probe label and are available with either an alkaline phosphatase (AP)/NBT-BCIP or horseradish-peroxidase (HRP)/AEC enzyme, substrate, chromogen combination, for your personal choice of detection.

Probe label
BIOTIN
DIGOXIGENIN
  Antibody conjugate
  Substrate Chromogen
Anti BIO-AP
NBT/BCIP
Anti BIO-HRP
Peroxide/AEC
Anti DIG-AP
NBT/BCIP
Anti DIG-HRP
Peroxide/AEC
  REMBRANDT® DNA in-situ
  hybridisation (DISH)
product code
product code
product code
product code
  Universal DISH kit
A001K.0105
A001K.0101
A001K.9905
A001K.9901
  Human Papilloma virus screening
A100K.0105
A100K.0101
A100K.9905
A100K.9901
  Human Papilloma virus typing
A103K.0105
A103K.0101
A103K.9905
A103K.9901
  Human cytomegalo virus
A200K.0105
A200K.0101
A200K.9905
A200K.9901
  Epstein-Barr virus
A300K.0105
A300K.0101
A300K.9905
A300K.9901
  Herpes simplex virus 1/2
A400K.0105
A400K.0101
A400K.9905
A400K.9901
  REMBRANDT® RNA in-situ
  hybridisation (RISH)
product code
product code
product code
product code
  Universal RISH kit
A000K.0105
A000K.0101
A000K.9905
A000K.9901
  Epstein-Barr early mRNAs (EBER)
A500K.0105
A500K.0101
A500K.9905
A500K.9901
  Kappa/Lambda light chain mRNAs
C600K.0105
C600K.0101
C600K.9905
C600K.9901

Universal in situ hybridisation and detection kits
The complete REMBRANDT® Universal ISH and Detection kits contain all reagents and materials for the preparation, pre-treatment and detection of labelled DNA or oligonucleotides by using the DNA (DISH) or RNA (RISH) in situ hybridisation technique. Positive and negative control probes are also included in these kits as positive and negative controls for the in situ hybridisation and detection procedure. The kits are offered for the detection of either biotin or digoxigenin labelled probes and are available with either an alkaline phosphatase (AP)/NBT-BCIP or horseradish-peroxidase (HRP)/AEC enzyme, substrate, chromogen combination, for your personal choice of detection.

BIO-AP
BIO-HRP
DIG-AP
DIG-HRP
: Biotin labelled probes combined with AP and NBT/BCIP detection
: Biotin labelled probes combined with HRP and AEC detection
: Digoxigenin labelled probes combined with AP and NBT/BCIP detection
: Digoxigenin labelled probes combined with HRP and AEC detection

The reagents in the kit have been optimised for use with probes labelled by the Universal Linkage System (ULS®). This labelling method results in probes that are unique in performance and reproducibility.

REMBRANDT® Universal ISH and Detection kits are therefore ideally suited for use in combination with one of our broad range of compatible labelled probes. Moreover, also home-made probes are perfectly suited for the listed Rembrandt® detection systems.

Furthermore, the DISH and especially RISH Detection kits can also be applied in routine immunohistochemistry for the detection of either biotin or digoxigenin labelled antibodies, thus serving different applications and cost saving.

IMPORTANT NOTICE
- The Universal DISH and Detection kits are supplied with PanWash differentiation reagent R013R.0000 for DNA probes with a GC% content < 50% (e.g. HPV).
- For the detection of DNA with a GC% content >50% (e.g. CMV, HSV and EBV), the appropriate PanWash differentiation reagent R014R.0000 is offered separately as listed in the Building Block section.
- The Rembrandt complete kits contain their specific PanWash differentiation reagent; either R013R.0000 or R014R.0000.
- The single DNA probe products are packaged with their specific differentiation reagent; either R013R.0000 or R014R.0000.
- The Universal RISH and Detection kits do not contain such PanWash differentiation reagents because the nature of oligonucleotides make such differentiation reagents obsolete.