Infections with herpes HSV are very common in humans and it is estimated that 90% of all humans experience oral herpes infections before the age of 10. HSV-1 causes an acute infection of gums, lips and sides of the mouth. HSV-2 causes infections in the genital region and affects 20-25% of the population and causes sores and blisters in the genital region. HSV is contagious and is transmitted by close contact (sexually transmitted) through active herpes blisters. HSV-1 establishes latency in the trigeminal ganglion whereas HSV-2 resides in the sacral ganglion. However, this difference is not absolute; either type can reside in either or both sites. HSV infections are usually mild. In some cases though, severe clinical manifestation of HSV infection may cause encephalitis, corneal blindness and it can lead to a spectrum of vesicular and necrotizing inflammatory conditions, mainly of the skin and mucous membranes, and disseminate in patients with secondary immunodeficiency syndromes. Both type 1 and 2 can cause intrauterine, neonatal and postnatal infections in newborns which has poor prognosis.
PanPath's REMBRANDT® kit for HSV is designed for the detection of both
types 1 and 2 since it is not necessary to distinguish between type 1 and
2 for diagnostic purposes. The kit therefore contains total genome DNA (153
Kb) probes for HSV type 1 and HSV type 2. No cross-hybridization occurs
with target DNA of other viruses provided that the hybridization conditions
as laid out in the protocol are strictly followed.